EZ-ASSIGN, a program for exhaustive NMR chemical shift assignments of large proteins from complete or incomplete triple-resonance data
نویسندگان
چکیده
منابع مشابه
De novo protein structure generation from incomplete chemical shift assignments.
NMR chemical shifts provide important local structural information for proteins. Consistent structure generation from NMR chemical shift data has recently become feasible for proteins with sizes of up to 130 residues, and such structures are of a quality comparable to those obtained with the standard NMR protocol. This study investigates the influence of the completeness of chemical shift assig...
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The NMR assignment of 13C, 15N-labeled proteins with the use of triple resonance experiments is limited to molecular weights below approximately 25,000 Daltons, mainly because of low sensitivity due to rapid transverse nuclear spin relaxation during the evolution and recording periods. For experiments that exclusively correlate the amide proton (1HN), the amide nitrogen (15N), and 13C atoms, th...
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ژورنال
عنوان ژورنال: Journal of Biomolecular NMR
سال: 2013
ISSN: 0925-2738,1573-5001
DOI: 10.1007/s10858-013-9778-y